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Immunofluorescent staining Cells were washed with PBS at 1000 rpm for 5 min twice, fixed with 4% paraformaldehyde for 2 h, then permeabilized with 0.1% Triton X-100 for 10 min at room temperature

The number of adhered PBMCs was counted with the ImageJ cell counter plugin

Collagen type I: Tng n hi ca da, gim np nhn v ci thin tnh trng ca tc v mng

Despite being extensively studied, current pharmacological and surgical interventions remain mostly symptomatic, leaving limited efficacy in halting or reversing disease progression

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